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A reversible effect of low carbon monoxide concentrations on the EPR spectra of the periplasmic hydrogenase from Desulfovibrio vulgaris
Authors:D S Patil  M H Czechowski  B H Huynh  J LeGall  H D Peck  D V DerVartanian
Institution:1. Department of Polymer Metrology & Technology, National Institute of Standards (NIS), Tersa Street, El Haram, P.O Box 136, Giza 12211, Egypt;2. Bioanalysis Laboratory, National Institute of Standards (NIS), Tersa Street, El Haram, P.O Box 136, Giza 12211, Egypt;3. Agricultural Engineering Department, Faculty of Agriculture, Cairo University, Giza, Egypt;4. Department of Biochemistry and Molecular Biology, Theodore Bilharz Research Institute, Giza, Egypt;5. Université Grenoble Alpes, CNRS, Grenoble INP, LGP2, Grenoble F-38000, France;6. Building Physics and Environment Institute, Housing and Building National Research Center (HBRC), Dokki, Giza, Egypt;7. Nanotechnology Research Center (NTRC), The British University in Egypt (BUE), El Sherouk City, Suez Desert Road, Cairo 11837, Egypt;1. UMR ECOSYS, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval Grignon, France;2. ADEME 20, Avenue du Grésillé, BP 90406, 49004 Angers Cedex 01, France;3. UR SAD, INRA, 88500 Mirecourt, France;4. CITEPA, 42 rue de Paradis, 75010 Paris, France;5. UMR Agronomie, INRA, AgroParisTech, Université Paris-Saclay, 78000 Thiverval Grignon, France
Abstract:The effect of low concentrations of CO (0.93 - 5.58 microM) on the EPR spectrum of the periplasmic non-heme iron hydrogenase from D. vulgaris has been investigated. The "g = 2.06" EPR signal is maximally induced (0.94 spin/molecule) at 46.5 microM CO and partial induction of the EPR signal could be observed at 0.93 microM CO. This effect is reversed by removal of the CO or irradiation of the hydrogenase with white light.
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