Contribution of L-alanine dehydrogenase to in vivo persistence and protective efficacy of the BCG vaccine |
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Authors: | Scandurra Gabriella M Ryan Anthony A Pinto Rachel Britton Warwick J Triccas James A |
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Institution: | Centenary Institute of Cancer Medicine and Cell Biology, Newtown, NSW 2042, Australia. |
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Abstract: | The tuberculosis (TB) vaccine strain Mycobacterium bovis BCG is unable to utilise alanine and this deficiency is thought to inhibit the growth of the vaccine in vivo and limit vaccine efficacy. In this report we demonstrate that L-alanine catabolism can be conferred on BCG by introduction of the gene encoding L-alanine dehydrogenase (Ald) of Mycobacterium tuberculosis. Restoration of Ald activity did not change the in vivo growth of BCG in macrophages or mice, and protection against aerosol M. tuberculosis infection was not altered by addition of ald to the BCG vaccine. These results demonstrate that the inability to utilise L-alanine is not a contributing factor to the attenuated phenotype of BCG and does not influence the protective efficacy of the vaccine against TB. |
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Keywords: | tuberculosis alanine dehydrogenase BCG vaccine virulence |
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