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Characterization and Diagnostic Use of a Monoclonal Antibody for VP28 Envelope Protein of White Spot Syndrome Virus |
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| Authors: | Chong-lin Hou Yu Cao Rong-hui Xie Yi-zhen Wang Hua-hua Du |
| Affiliation: | Chong-lin Hou1,Yu Cao1,Rong-hui Xie2,Yi-zhen Wang1 and Hua-hua Du1(1.Key Laboratory of Molecular Animal Nutrition,Ministry of Education,Feed Science Institute,Zhejiang University,Hangzhou 310029,China,2.Zhejiang Provincial Center for Disease Prevention and control,China) |
| Abstract: | The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV) was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21.After induction,the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal antibody (MAb) production.It was observed by immuno-electron microscopy the MAbs specific to rVP28 could recognize native VP28 target epitopes of WSSV and dot-blot analysis was used to detect natural WSSV infection.Co... |
| Keywords: | White spot syndrome virus (WSSV) Recombinant VP28 Monoclonal antibody (MAb) |
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