| 利用响应面方法优化转小鼠金属硫蛋白-I基因聚球藻7002的培养基成分 |
| |
| 引用本文： | 曾文炉,赵飞飞,曹照根,茹炳根. 利用响应面方法优化转小鼠金属硫蛋白-I基因聚球藻7002的培养基成分[J]. 生物工程学报, 2008, 24(1): 130-136 |
| |
| 作者姓名： | 曾文炉 赵飞飞 曹照根 茹炳根 |
| |
| 作者单位： | 1. 北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京,100871;南开大学环境科学与工程学院,天津市城市生态环境修复与污染防治重点实验室,天津,300071
2. 北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京,100871 |
| |
| 基金项目： | 国家第三十三批博士后科学基金(No. 2003033079)。 本项研究得到的结果, 其知识产权归北京大学所有。 |
| |
| 摘 要： | 为实现转小鼠金属硫蛋白基因-I聚球藻7002的高密度培养, 并将其应用于实际的重金属废水处理过程, 首先需要对培养基的成分进行优化。本文利用响应面这一多因素过程优化的有效工具, 通过全因子实验、最陡爬坡实验和中心组合实验, 对转小鼠金属硫蛋白基因-I聚球藻7002培养基的主要成分以及初始pH进行了优化。优化后的培养基组成为: NaHCO3 1.696 g/L, NaNO3 8.57 g/L, 初始pH为8.57, 其他成分同Medium A。优化条件分别在2 L和20 L气升式光生物反应器中得到了验证, 最大细胞浓度分别达到每升4.16 g干重和每升3.12 g干重, 分别比优化前提高了9倍和7倍, 从而为其产业化应用打下基础。
|
| 关 键 词： | 小鼠金属硫蛋白-I 聚球藻7002 转基因 响应面方法 优化 |
| 收稿时间： | 2007-04-03 |
| 修稿时间： | 2007-06-04 |
Medium Optimization by Response Surface Method for Transgenic Synechococcus sp. PCC 7002 with Mouse Metallothionein-I Gene |
| |
| Wenlu Zeng,Feifei Zhao,Zhaogen Cao and Binggen Ru. Medium Optimization by Response Surface Method for Transgenic Synechococcus sp. PCC 7002 with Mouse Metallothionein-I Gene[J]. Chinese journal of biotechnology, 2008, 24(1): 130-136 |
| |
| Authors: | Wenlu Zeng Feifei Zhao Zhaogen Cao Binggen Ru |
| |
| Affiliation: | 1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China; 2 College of Environmental Science and Engineering, Tianjin Key Laboratory of Environmental Remediation and;1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;1 The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China |
| |
| Abstract: | To implement high cell density cultivation of transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene, and its applications in heavy metal wastewater, we optimized the medium components. We used response surface method, a useful tool for multi-factor process optimization, through a three- step experiment design: full factor design, the steepest climbing and central composite design. The selected three factors were NaHCO3, NaNO3, and initial pH. The optimized medium contained 1.696 g/L NaHCO3 and 8.57 g/L NaNO3, at initial pH 8.57. The optimized medium was validated in 2-L and 20-L air lift photo-bioreactor, respectively. The maximum cell concentration reached 4.16 g/L for 2-L bioreactor, and 3.12 g/L for 20-L bioreactor. |
| |
| Keywords: | mouse metallothionein-I Synechococcus sp. PCC 7002 transgenic response surface method optimization |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
|
点击此处可从《生物工程学报》下载全文 |
|
