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Saccharomyces cerevisiae actin--Escherichia coli lacZ gene fusions: synthetic-oligonucleotide-mediated deletion of the 309 base pair intervening sequence in the actin gene |
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| Authors: | G P Larson K Itakura H Ito J J Rossi |
| Affiliation: | Department of Molecular Genetics, City of Hope Research Institute, 1450 E. Duarte Road, Duarte, CA 91010 U.S.A. Tel. (213) 357-9711 |
| Abstract: | |
| Keywords: | Recombinant DNA oligonucleotide-directed in vitro mutagenesis RNA splicing and processing introns exons colony screening β-galactosidase shuttle plasmids autonomously replicating sequence bp base pairs CCC covalently-closed circular EtBr ethidium bromide IVS intervening sequence (intron) kb kilobase pairs SD see MAT. AND METH. (section b) SDS sodium dodecyl sulfate SET see MAT. AND METH. (section k) SSC Xgal YEPD see MAT. AND METH. (section b): Δ, deletion |
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