Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim |
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| Authors: | Bin Guo Yong-Gang Liu Qiong Yan Chun-Zhao Liu |
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| Affiliation: | (1) National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P. R. China;(2) College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100093, P. R. China;(3) Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USA;(4) Graduate School of the Chinese Academy of Sciences, Beijing, 100049, P. R. China |
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| Abstract: | Cell growth, flavonoids biosynthesis and L-phenylalanine ammonia-lyase (PAL) activity were studied in callus cultures of Saussurea medusa Maxim. under different types of spectral radiance. After 21 days, red light significantly improved the callus growth, but inhibited the biosynthesis of flavonoids in callus cultures. However, blue light was found to enhance the biosynthesis of flavonoids, although callus growth under this spectrum was comparable with that under white and other coloured spectra, such as green and yellow. The accumulation of flavonoids in callus cultures was related to the PAL activity, which was found to be stimulated by the spectral composition of irradiation. |
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| Keywords: | Callus culture Cell growth Flavonoids biosynthesis Spectral radiance Saussurea medusa |
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