Virus-Specific Deoxyribonucleic Acid in Simian Virus 40-Exposed Hamster Cells: Correlation with S and T Antigens |
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| Authors: | Arthur S Levine Michael N Oxman Patrick H Henry Myron J Levin George T Diamandopoulos and John F Enders |
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| Institution: | Medicine Branch, National Cancer Institute, Bethesda, Maryland 20014;Research Division of Infectious Diseases and Children''s Cancer Research Foundation, Children''s Hospital Medical Center, Boston, Massachusetts 02115;Departments of Bacteriology and Immunology, and Pathology, Harvard Medical School, Boston, Massachusetts 02115 |
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| Abstract: | Several homologous hamster embryonic cell lines, transformed in association with simian virus (SV) 40 infection, were examined for the presence of deoxyribonucleic acid (DNA) complementary to SV40 ribonucleic acid (RNA) made in vitro. The methods employed permitted the detection of 10(-5) mug of viral DNA in 100 mug of cellular DNA, corresponding to one-fifth of an SV40 DNA molecule per cell. Those lines which contained both the SV40 surface (S) and tumor (T) antigens also contained DNA complementary to SV40 RNA synthesized in vitro. In contrast, neither of two lines which contained S, but not T, antigen contained detectable DNA complementary to SV40 RNA. These findings suggest that the production of S antigen does not depend upon the persistence of SV40 DNA in transformed cells. |
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