A novel approach and a fully automated microcomputer-based system to study kinetics of NO−3, NO−2, and NH+4 transport simultaneously by intact wheat seedlings |
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| Authors: | S. S. GOYAL R. C. HUFFAKER |
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| Affiliation: | Plant Growth Laboratory, Department of Agronomy &Range Science, University of California, Davis, CA 95616, U.S.A. |
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| Abstract: | Abstract A 16-channel fully automated microcomputer-based system was designed to measure the disappearance of NO?3 NO?2 and NH+4 simultaneously from uptake solutions. The analyses were done using high-performance liquid chromatography. Statistical procedures were used to generate transport kinetics and interactions amongst NO?3, NO?2 and NH+4 by intact wheat seedlings. The simultaneous analysis of NO?3, NO?2 and NH+4 at real-time; the accommodation of varying sampling intervals; the capability to study up to 16 experimental units in synchrony; and the analysis of the data with a microcomputer, make this a powerful system for studying transport kinetics and interactions. |
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| Keywords: | N uptake kinetics microcomputer ion transport wheat seedlings |
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