Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae |
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Authors: | G. J. Scoles B. S. Gill Z. -Y. Xin B. C. Clarke C. L. McIntyre C. Chapman R. Appels |
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Affiliation: | (1) Division of Plant Industry, CSIRO, P.O. Box 1600, 2601 Canberra, ACT, Australia;(2) Dept. of Crop Science and Plant Ecology, University of Saskatchewan, S7N OWO Saskatoon, Canada;(3) Dept. of Plant Pathology, Kansas State University, 66506 Manhattan, KS, USA;(4) Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing, China;(5) International Board for Genetic Resources, FAO, 20437 Washington, DC, USA |
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Abstract: | The 5 S DNA units from 15 grasses in theTriticeae were analysed at the DNA sequence level. Four units carried duplications near the 3-end of the 5 S RNA gene with 3 of the duplications centred on the same base pairs as a duplication previously reported byGerlach & Dyer. The fourth duplication was located 3 downstream from the gene, in the spacer region. Apparent deletions were very frequent when units of the different grasses were compared and it was clear that these deletions did not extend into a 75 bp spacer region upstream from the 5 S RNA gene. This 75 bp region also tended to be more conserved between the grasses as compared to the high level of sequence change in the rest of the spacer region. — Phenetic relationships were established between the grasses using the sequence data. The relationships were generally consistent with the data from other parameters and, in addition, showed that two Australian grasses were closely related to the other Northern hemisphere genera examined. The data concerning the Australian grasses is discussed in relation to the isolated nature of Australia. |
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Keywords: | Angiosperms Poaceae Triticeae 5 S DNA sequence analyses duplication and deletion events phenetic relationships |
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