Copper exposure impairs intra- and extracellular acid-base regulation during hypercapnia in the fresh water rainbow trout (Oncorhynchus mykiss) |
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Authors: | T Wang P K Knudsen C J Brauner M Busk M M Vijayan F B Jensen |
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Institution: | (1) Centre for Respiratory Adaptation, Institute of Biology, Odense University, DK-5230 Odense M, Denmark e-mail: wang@biology.ou.dk, Tel.: +45-65-57-24-60, Fax: +45-65-93-04-57, DK |
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Abstract: | In order to evaluate the impact of water-borne copper on acid-base regulation in fresh water rainbow trout, chronically cannulated
fish were exposed to copper (0.6 mg 1−1), hypercapnia (water PCO2 of 6 mmHg) or a combination of copper and hypercapnia, while a fourth untreated group served as the control. Blood samples
obtained at 0 h, 4 h and 24 h were analysed for acid-base status, ion concentrations and respiratory parameters. Tissue samples
from caudal skeletal muscle, liver and gill filaments were examined for intracellular acid-base status, ion- and water contents,
and copper concentration. Exposure to copper alone elicited a small extracellular metabolic alkalosis, no changes in arterial
PO2, and a minor decrease in plasma ion concentrations. Hypercapnia alone increased arterial PCO2 from approximately 2 mmHg to 7.2 mmHg, but the extracellular respiratory acidosis present at 4 h was almost completely compensated
at 24 h due to an increase in plasma bicarbonate concentration HCO3
−] from 8.1 mM to 24.4 mM. Combined exposure to hypercapnia and copper resulted in a slightly larger acidosis at 4 h, and the
fish failed to restore extracellular pH at 24 h, because plasma HCO3
−] only increased to 16.3 mM. Fish exposed to hypercapnia and copper also showed a delayed recovery of intracellular pH in
skeletal muscle, compared to fish exposure to hypercapnia only. Thus, copper exposure impaired both extracellular and intracellular
acid-base regulation during hypercapnia. When seen in connection with only minor effects of copper on osmoregulatory and respiratory
parameters, the reduced ability to regulate acid-base suggests that acid-base regulation may be one of the most copper-sensitive
branchial functions.
Accepted: 18 August 1998 |
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Keywords: | Hypercapnia Acid-base balance Ion regulation Blood gases Copper |
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