The constitutive centromere component CENP-50 is required for recovery from spindle damage |
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Authors: | Minoshima Yukinori Hori Tetsuya Okada Masahiro Kimura Hiroshi Haraguchi Tokuko Hiraoka Yasushi Bao Ying-Chun Kawashima Toshiyuki Kitamura Toshio Fukagawa Tatsuo |
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Institution: | National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. |
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Abstract: | We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in vertebrate cells. To determine the precise role of CENP-50, we examined its role in centromere function by generating a loss-of-function mutant in the chicken DT40 cell line. The CENP-50 knockout was not lethal; however, the growth rate of cells with this mutation was slower than that of wild-type cells. We observed that the time for CENP-50-deficient cells to complete mitosis was longer than that for wild-type cells. Centromeric localization of CENP-50 was abolished in both CENP-H- and CENP-I-deficient cells. Coimmunoprecipitation experiments revealed that CENP-50 interacted with the CENP-H/CENP-I complex in chicken DT40 cells. We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage. |
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