| The rat glomerular and tubular guanylate cyclase in normal and nephrotic kidney: is a membrane-bound effector involved in the modulation of the rat enzyme? |
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| 摘 要： |
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The rat glomerular and tubular guanylate cyclase in normal and nephrotic kidney: Is a membrane-bound effector involved in the modulation of the rat enzyme? |
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| J J Helwig,A N Yusufi,J Geisert,P Mandel,G Rebel. The rat glomerular and tubular guanylate cyclase in normal and nephrotic kidney: Is a membrane-bound effector involved in the modulation of the rat enzyme?[J]. The International journal of biochemistry, 1981, 13(7): 783-789. DOI: 10.1016/0020-711X(81)90096-3 |
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| Authors: | J J Helwig A N Yusufi J Geisert P Mandel G Rebel |
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| Affiliation: | 1. Hospital Universitario de la Princesa, Servicio de Neumología, Madrid, Spain;2. Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain;3. Centro de Investigación en Red de Enfermedades Respiratorias, Instituto de Salud Carlos III, Madrid, Spain;4. Division of Critical Care, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, Canada;5. Department of Surgery, University of Toronto, Toronto, Canada;6. World Health Organization, Geneva, Switzerland;1. School of Mechanical Engineering, Jiangnan University, Wuxi, China;2. Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi, China;3. Department of Electrical and Computer Engineering, Western University, London, Canada;4. School of Microelectronics, Tianjin University, Tianjin, China;1. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, China;2. Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;3. Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019-5300, United States;4. School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China;5. Guangdong Key Laboratory of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou 510006, China;1. Instituto de Botânica, Núcleo de Pesquisa Em Ecologia, PO Box 68041, 04045-972, SP, Brazil;2. Instituto de Biociências – USP, Brazil;3. Secretaria de Estado Do Meio Ambiente SEMA-PA, Brazil;4. Centro Universitário Adventista de São Paulo, UNASP, Brazil;5. Centro de Capacitação e Pesquisa Em Meio Ambiente, CEPEMA, POLI, USP, Brazil;1. Energy Resources Eng. Depart., Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt;2. Mech. Power Eng. Depart. Faculty of Eng., Tanta University, Tanta, Egypt;3. Mech. Power Eng. Depart. Faculty of Eng., Benha University, Benha, Egypt;4. Mech. Eng. Depart. Faculty of Eng., Assiut University, Assiut, Egypt;1. Programa de Enfermedades Neuromusculares y Trastornos Motores, Departamento de Neurología Pediátrica, Clínica Las Condes, Santiago, Chile;2. Policlínico Neuromuscular, Unidad de Neurología Pediátrica, Instituto Nacional de Rehabilitación Infantil Pedro Aguirre Cerda, Santiago, Chile |
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| Abstract: | - 1.1. Homogenization and ultrasonication of purified glomeruli from normal rat kidney led respectively to a 90 and 70% loss of the guanylate cyclase activity (GCase). Cell fractionation showed a soluble enzyme enrichment and an inhibiting effect of the 105,000 g pellet against intact glomeruli and soluble enzymes.
- 2.2. In rabbit glomeruli, neither loss of GCase activity by cell disruption nor inhibitor were detected.
- 3.3. GCase from nephrotic rats (12 days after puromycin aminonucleoside injection) was not more or less affected by these means of cell disruption. The inhibiting effect was also present.
- 4.4. Identical phenomena were found in the purified tubules but to a much lesser degree than in the glomeruli.
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