| 牛眼透明质酸的分离及性质测定 |
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| 引用本文： | 罗曼,蒋立科,奚俊.牛眼透明质酸的分离及性质测定[J].生物化学与生物物理进展,1999,26(6):596-600. |
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| 作者姓名： | 罗曼 蒋立科 奚俊 |
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| 作者单位： | 安徽农业大学生物工程系，合肥 230036;安徽农业大学生物工程系，合肥 230036;合肥神鹿药业有限公司，合肥 230036 |
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| 基金项目： | 安徽省重点科研项目资助. |
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| 摘 要： | 研究了牛眼透明质酸(HA)的提纯方法,采用0.125 mol/L Na2SO4取代0.1 mol/L NaCl溶解粗品，发现前者溶解液中有2个组分，一个组分被十六烷基溴代吡啶(CBP)吸附回收，产物呈纤维状，另一组分保留在溶液中并能通过分级稀释而被沉淀回收，产物呈粉末状；而用0.1 mol/ml NaCl溶解粗品，除了被CBP吸附回收外，保留在溶液中的HA不能被回收；与同类方法相比，回收率提高1.7倍以上.还分析了产物的性质，与同类样品比较，提高了纯度，降低了提纯中有机溶剂的消耗.
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| 关 键 词： | 牛眼，透明质酸，分级分离，理化性质，质量 |
| 收稿时间： | 1998/11/9 0:00:00 |
| 修稿时间： | : 1998-11- |
A Study on the Separation and Character of Hyaluronic Acid from Ox Eyes |
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| LUO Man,JIANG Li-Ke and XI Jun.A Study on the Separation and Character of Hyaluronic Acid from Ox Eyes[J].Progress In Biochemistry and Biophysics,1999,26(6):596-600. |
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| Authors: | LUO Man JIANG Li-Ke and XI Jun |
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| Institution: | Anhui Agriculture University, Hefei 230036, China;Anhui Agriculture University, Hefei 230036, China;Hefei Shenlu Medicineslimited Company, Hefei 230036, China |
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| Abstract: | A method for purifying HA from ox eyes was studied.Using 0.125 mol/L Na2SO4 other than 0.1 mol/L NaCl to dissolve the semifinished product, Two HA forms in Na2SO4's solution were found. One in staple could be absorbed by CBP, and the other in powder could be returned by fractional dilution afterwards. In 0.1 mol/L NaCl solution, HA could only be recovered by CBP absorption. It displayed staple. The recoverable rate of the method was 1.7 times than others.The physical and chemical characters of the product were analysed. It was proved that higher purity recovery and lower cost than other methods were gained. |
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| Keywords: | ox eye hyaluronic acid fractionation physical and chemical character quality |
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