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Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease
Affiliation:1. Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands;2. Leiden Institute of Physics, Leiden University, 2333 Leiden, the Netherlands;3. Center for Proteomics and Metabolomics, Leiden University Medical Center, 2333 Leiden, the Netherlands;4. Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 Leiden, the Netherlands;5. Sequencing Analysis Support Core, Leiden University Medical Center, 2333 Leiden, the Netherlands;6. Centro de Biologia Molecular Severo Ochoa, Departamento de Física de la Materia Condensada, Instituto Nicolas Cabrera and Condensed Matter Physics Center (IFIMAC), Universidad Autónoma de Madrid, 28049 Madrid, Spain;7. Vascular Biology and Regenerative Medicine Unit, Centro Cardiologico Monzino IRCCS, 20138 Milan, Italy;8. Department of Clinical Sciences and Community Health, Università degli Studi di Milano, 20122 Milan, Italy;9. Central Laboratory Animal Facility, Leiden University Medical Center, 2333 Leiden, the Netherlands;10. Department of Epidemiology and Biostatistics, Amsterdam Public Health Institute, VU University Medical Center, 1007 Amsterdam, the Netherlands;11. Department of Cardiology, Leiden University Medical Center, 2333 Leiden, the Netherlands;12. Department of Biology, University of Padua, 35121 Padua, Italy;13. Veneto Institute of Molecular Medicine, 35129 Padua, Italy;14. Department of Applied Stem Cell Technologies, University of Twente, 7500 Enschede, the Netherlands
Abstract:
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  • Keywords:human-induced-pluripotent-stem-cell-derived cardiomyocytes  human-induced-pluripotent-stem-cell-derived cardiac fibroblasts  cardiac microtissue  cardiomyocyte maturation  cell-cell interaction  gap junction  cyclic AMP  cAMP  arrhythmogenic cardiomyopathy  cardiac disease model
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