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Comparison of K-Cl cotransport expression in high and low K dog erythrocytes.
Authors:Hideharu Ochiai  Kazunari Higa  Shin Hisamatsu  Hiroshi Fujise
Affiliation:Research Institute of Biosciences, Azabu University, Sagamihara, Kanagawa, Japan.
Abstract:K-Cl cotransport plays a crucial role in regulatory volume decrease of erythrocytes. K-Cl cotransport activities in dog erythrocytes with an inherited high Na-K pump activity (HK) and normal erythrocytes (LK) were compared. Nitrite (NO(2)) stimulated K-Cl cotransport activity in HK cells around 14-fold at 2.4 mM, and it also increased the Km value of this cotransporter. Real-time PCR and western blot analysis revealed that K-Cl cotransporter 1 was dominant, and that the quantity of K-Cl cotransporter 1 protein was comparable between HK and LK erythrocytes. These results suggest that the difference in cotransport activity was not caused by the amount of K-Cl cotransport protein but by a difference in the regulation system, which is susceptible to oxidant.
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