Bone resorption and prostaglandin production by mouse calvaria in vitro: Response to exogenous prostaglandins and their precursor fatty acids |
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Authors: | J.M. Katz T. Wilson S.J.M. Skinner D.H. Gray |
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Affiliation: | 1. Department of Surgery, School of Medicine, University, of Auckland, Auckland, New Zealand;2. Postgraduate School of Obstetrics & Gynaecology, University of Auckland, Auckland, New Zealand |
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Abstract: | Mouse calvaria were maintained in organ culture for 96 h and endogenous prostaglandin production and active bone resorption (45 Ca release) measured. After a lag phase of 12 h, active resorption increased over the 96 h period. The amounts of prostaglandins released into the culture medium (measured by radioimmunoassay) were highest in the first 24 h of culture. Unless these were removed by preculturing for 24 h, or suppressed by indomethacin, no response to exogenous PGE2, PGF2α or prostaglandin precursors could be demonstrated. Bone resorption was stimulated after preculture by both PGE2 and PGF2α in a dose-dependent manner (10?18M – 10?5M), with PGE2 being the more potent. Collagen synthesis was unaffected by PGF2α, whereas PGE2 (10?5M) had an inhibitory effect. Eicosatrienoic acid did not stimulate bone resorption at lower concentrations (10?7M – 10?5M_, but was inhibitory at 10?4M. Arachidonic acid also inhibited resorption at 10?4M, but at lower concentrations (10?7M – 10?5M0 increased active resorption. This was concomitant with a rise in PGE2 and PGF2α levels, PGE2 production being significantly higher than PGF2α. The effects of PGE2 (10?8M) and PGF2α (10∞M appeared additive: there was no evidence of synergistic or antagonistic effects when varying ratios of PGE2 : PGF2α were employed. |
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