Studies on phosphorylation coupled with denitrification and aerobic respiration inPseudomonas denitrificans |
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Authors: | Hisayoshi Terai Takeshi Mori |
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Institution: | 1. Biological Institute, Faculty of Science, Nagoya University, 464, Nagoya
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Abstract: | Formation of ATP during aerobic respiration and denitrification was determined inPseudomonas denitrificans. In the intact cell system, the ATP formation associated with denitrification was almost the same as that associated with aerobic respiration when lactate was used as an electron donor. The ATP formation was inhibited by KCN, NaN3 and DNP. No phosphate uptake occurred when NH2OH, DMPD or TMPD was used as an electron donor, although the production of N2O, N2 or NO from nitrite was accelerated under anaerobic conditions. In the cell-free system, the ATP formation was also demonstrated using an ATP trapping system and lactate as a substrate. The effects of inhibitors were almost the same as those observed with the intact cells. DMPD or TMPD together with ascorbate promoted the ATP formation during aerobic oxidation by the cell-free system whereas no stimulation of ATP formation was detected during denitrification. |
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