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Factors affecting heterologous gene expression in Saccharomyces cerevisiae |
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| Authors: | J Mellor M J Dobson N A Roberts A J Kingsman S M Kingsman |
| Affiliation: | Department of Biochemistry, South Parks Road, Oxford, OX1 3QU U.K. Tel. Oxford 511261, Ext. 239 |
| Abstract: | |
| Keywords: | Yeast PGK promoter IFN protein mRNA levels and start point mapping terminator bp base pairs DTT dithiothreitol EtBr ethidium bromide IFN interferon IU international units kb kilobase pairs MOPS PAGE polyacrylamide gel electrophoresis PGK phosphoglycerate kinase PMSF phenyl methyl sulfonyl fluoride SDS sodium dodecyl sulphate YPD yeast extract peptone dextrose medium |
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