A flexible single-step detection of blotted antigen using a fusion protein between protein A and green fluorescent protein |
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| Authors: | Aoki T Miyashita M Fujino H Watabe H |
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| Affiliation: | Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. aokit@hoku-iryo-u.ac.jp |
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| Abstract: | A green fluorescent protein mutant (S147P GFP) was fused with protein A and expressed in Escherichia coli. This fusion protein (PA-GFP147) was used in immunoblotting studies as a new detection system, designated as "flexible single-step detection (FSSD)". In FSSD, the detection of blotted antigen was done in one step, and the antigen-antibody reaction can be monitored by UV-irradiation in real time. The reaction time, therefore, can be flexibly controlled by monitoring the green fluorescence. |
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