Analysis of Mycelial Growth Rates and RAPD-PCR Profiles in a Population of Gaeumannomyces graminis var. tritici Originating from Wheat Plants Grown from Fungicide-treated Seed |
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| Authors: | Z. Weber L. Irzykowska J. Bocianowski |
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| Affiliation: | Authors' addresses: Department of Phytopathology, University of Agriculture, Dąbrowskiego 159, 60–594 Poznań, Poland;;Department of Mathematical and Statistical Methods, University of Agriculture, Wojska Polskiego 28, 60–637 Poznań, Poland (correspondence to Z. Weber. E-mail: ) |
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| Abstract: | Linear mycelial growth rates of 70 isolates of Gaeumannomyces graminis var. tritici on agar medium amended or unamended with the fungicide silthiofam were not correlated. Mycelial growth rate was not influenced by the fungicide applied to the seed of the plants from, which the isolates originated. DNA polymorphism determined by randomly amplified polymorphic DNA (RAPD) polymerase chain reaction was used to assess genetic variation among isolates. Thirty RAPD markers generated with five arbitrary 10‐mer primers revealed DNA polymorphism suitable for assessing variability in this fungal population. Cluster analysis of RAPD data identified two groups at the 54% similarity level. There was a significant relationship between the presence of 11 markers and sensitivity to silthiofam. |
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| Keywords: | fungicide Gaeumannomyces graminis RAPD-PCR take-all |
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