| 小球藻糖蛋白的分离、纯化和结构分析 |
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| 引用本文： | 李亚娜,林永成,佘志刚,周利平. 小球藻糖蛋白的分离、纯化和结构分析[J]. 天然产物研究与开发, 2004, 16(6): 503-506 |
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| 作者姓名： | 李亚娜 林永成 佘志刚 周利平 |
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| 作者单位： | 1. 中山大学化学博士后流动站,广州,510275
2. 湖南省科技厅,长沙,410000 |
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| 基金项目： | 国家自然科学基金项目 (3 0 2 0 0 2 2 6) |
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| 摘 要： | 采用柱层析分离纯化小球藻糖蛋白，并对其进行结构分析。结果表明，小球藻糖蛋白为白色粉末，产率为鲜藻的0．28％，易溶于水，分子量约为65，000Da，属O-连接方式，蛋白质含量为12．01％，糖链部分含有岩藻糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖。
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| 关 键 词： | 糖蛋白 纯化 半乳糖 糖链 葡萄糖 甘露糖 分离 小球藻 分子量 连接方式 |
| 修稿时间： | 2004-04-22 |
ISOLATION,PURIFICATION AND STRUCTURE DETERMINATION OF CHLORELLA GLYCOPROTEIN |
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| LI Ya-na ,LIN Yong-cheng,SHE Zhi-gang,ZHOU Li-ping. ISOLATION,PURIFICATION AND STRUCTURE DETERMINATION OF CHLORELLA GLYCOPROTEIN[J]. Natural Product Research and Development, 2004, 16(6): 503-506 |
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| Authors: | LI Ya-na LIN Yong-cheng SHE Zhi-gang ZHOU Li-ping |
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| Affiliation: | LI Ya-na 1*,LIN Yong-cheng1,SHE Zhi-gang1,ZHOU Li-ping2 |
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| Abstract: | CPG was isolated and purified by column chromatography.Then its structure was studied.The results showed that CPG is the white solid powder and easily dissolved in water.Productivity is 0.28%(wet weight).The molecular weight is 65,000 Da.The linkage between glycan and core protein backbone is O-linkage.The protein content was up to 12.01%.The saccharide chain was composed of fucose,xylose,mannose,glucose,galactose. |
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| Keywords: | CPG isolation purification structure determination |
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