Pulsed-Field Gel Electrophoresis Is More Discriminating Than Multilocus Enzyme Electrophoresis and Random Amplified Polymorphic DNA Analysis for Typing Pyogenic Streptococci |
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Authors: | Frédéric Bert Catherine Branger Nicole Lambert-Zechovsky |
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Institution: | H?pital Beaujon, service de Microbiologie, 100 boulevard du
Général Leclerc, 92210 Clichy, France, FR
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Abstract: | The SmaI restriction endonuclease digestion
patterns of chromosomal DNAs from 99 pyogenic streptococci belonging to
Lancefield group A (41 Streptococcus pyogenes), group C (seven S.
dysgalactiae, 11 \QS. equisimilis\W, three S. equi, eight
S. zooepidemicus) and group G (25 human group G
Streptococcus, four S. canis) were analyzed by pulsed-field
gel electrophoresis (PFGE), and the results were compared with those
previously obtained by multilocus enzyme electrophoresis (MLEE) and random
amplified polymorphic DNA analysis (RAPD). PFGE revealed 93 distinct types
among the 99 strains, and no patterns were common to strains of different
species. The discriminatory power of PFGE was greater than that of MLEE and
RAPD for groups A and G streptococci. The polymorphism among group C
streptococci was similar with the three techniques. PFGE is, therefore, the
most efficacious method for epidemiological typing of pyogenic streptococci.
Received: 31 July 1996 / Accepted: 17 August 1996 |
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