Transduced Tat-SOD fusion protein protects against ischemic brain injury |
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Authors: | Kim Dae Won Eum Won Sik Jang Sang Ho Kim So Young Choi Hee Soon Choi Soo Hyun An Jae Jin Lee Sun Hwa Lee Kil Soo Han Kyuhyung Kang Tae-Cheon Won Moo Ho Kang Jung Hoon Kwon Oh-Shin Cho Sung-Woo Kim Tae Yoon Park Jinseu Choi Soo Young |
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Institution: | Department of Genetic Engineering and Research, Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea. |
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Abstract: | Reactive oxygen species (ROS) are implicated in reperfusion injury after transient focal cerebral ischemia. The antioxidant enzyme, Cu,Zn-superoxide dismutase (SOD), is one of the major means by which cells counteract the deleterious effects of ROS after ischemia. Recently, we reported that when Tat-SOD fusion protein is transduced into pancreatic beta cells it protects the beta cells from destruction by relieving oxidative stress in ROS-implicated diabetes (Eum et al., 2004). In the present study, we investigated the protective effects of Tat-SOD fusion protein against neuronal cell death and ischemic insults. When Tat-SOD was added to the culture medium of neuronal cells, it rapidly entered the cells and protected them against paraquat-induced cell death. Immunohistochemical analysis revealed that Tat-SOD injected intraperitoneally (i.p.) into mice has access to various tissues including brain neurons. When i.p. injected into gerbils, Tat-SOD prevented neuronal cell death in the hippocampus in response to transient fore-brain ischemia. These results suggest that Tat-SOD provides a strategy for therapeutic delivery in various hu-man diseases, including stroke, related to this anti-oxidant enzyme or to ROS. |
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