Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells |
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Authors: | Hunt L Fletcher J S |
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Affiliation: | Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73069. |
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Abstract: | The amount of carbon (μmoles of carbon atoms) drained from the tricarboxylic acid cycle for protein synthesis was compared with μmoles of CO2 released from the cycle at 2-day intervals during the growth of suspension cultures of Paul's Scarlet rose. We concluded that during the period of most rapid protein synthesis (day 0-4) one-sixth as much carbon was drained from the tricarboxylic acid cycle for protein synthesis as was released as CO2. By day 8, one-thirtieth of the amount of carbon released as CO2 was incorporated into protein. Net protein synthesis stopped on day 8, but the evolution of CO2/culture continued at its maximum rate until day 10. |
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