| 用ESR自旋捕集法研究吸烟烟气处理的脂质体对大鼠粒细胞产生O_2~-的影响 |
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| 引用本文： | 杨法军,赵保路,忻文娟. 用ESR自旋捕集法研究吸烟烟气处理的脂质体对大鼠粒细胞产生O_2~-的影响[J]. 生物物理学报, 1992, 0(4) |
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| 作者姓名： | 杨法军 赵保路 忻文娟 |
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| 作者单位： | 中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所 北京 100101，北京 100101，北京 100101 |
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| 摘 要： | 吸烟烟气能引发卵磷脂脂质体的脂质过氧化。若将吸烟烟气处理20s的脂质体作用完整的大鼠粒细胞(RPN),用ESR自旋捕集方法发现,当作用时间在25min内(脂浓度1.0mg/ml)或脂浓度小于15.0mg/ml(而作用时间均为15min)时,这种过氧化的脂质体能增加RPN呼吸爆发产生O_2~-的量。而没有经吸烟烟气处理的新制脂质体,在脂浓度大于0.2mg/ml(作用时间15min)时,都不同程度地抑制RPN产生O_2~-。
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| 关 键 词： | ESR自旋捕集 粒细胞 呼吸爆发 脂质过氧化 |
ESR STUDIES ON THE EFFECTS OF GPCS TREATED LIPOSOMES ON SUPEROXIDE-GENERAIDN OF RAT NEUTROPHILS |
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| Yang Fajun Zhao Baolu Xin Wenjuan. ESR STUDIES ON THE EFFECTS OF GPCS TREATED LIPOSOMES ON SUPEROXIDE-GENERAIDN OF RAT NEUTROPHILS[J]. Acta Biophysica Sinica, 1992, 0(4) |
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| Authors: | Yang Fajun Zhao Baolu Xin Wenjuan |
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| Abstract: | Gas-phase cigarette smoke (GPCS) was capable of inducing lipid peroxid-ation in lecithin-formed liposomes. When intact rat peritoneal neutroghils (RPN) were treated by the liposomes which had been bubbled by GPCS for 20 seconds, it was found, by using ESR spin trapping technique, that these pe-roxidized lipids enhanced the generation of superoxide anions in RPNs, if the duration time was within 25 minutes (1.0 mg/ml lipids) or the concentration of lipids was less than 15.0 nig/nil (the duration time was 15 minutes). While, the newly-prepared liposomes without GPCS-treating showed an inhibiting effect on the activity of RPNs when the concentration of lipids was more than 0.2mg/ml (the duration time was 15 minutes). |
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| Keywords: | ESR spin trapping neutrophil respiratory burst lipid peroxidation. |
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