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Lysyl tRNAs of lupinus luteus seeds
Authors:H. Augustyniak  J. Pawełkiewicz
Affiliation:Institute of Biochemistry of Poznań Universities, 60-637 Poznań, Poland
Abstract:Lysine accepting transfer RNA of lupin seeds and lupin embryo axes can be fractionated into at least 5 species by reversed-phase chromatography (RPC-5). One main and two minor isoacceptors were observed in wheat and barley embryos. Changes in isoaccepting species of tRNA1ys were followed in cotyledons of germinating lupin seedlings. Ribosome binding studies revealed that one of the main lupin tRNA1ys species recognizes the AAG codon, the second AAA and the third one AAA and AAG.
Keywords:Leguminosae  lysyl-transfer RNA isoacceptors.
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