Seed glycoproteins of Lupinus angustifolius |
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Authors: | Christopher A. Eaton-Mordas Keith G. Moore |
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Affiliation: | 1. School of Plant Biology, University College of N. Wales, Bangor, U.K.;2. School of Biological Sciences, Univeristy of Bath, Claverton Down, Bath BA2 7AY, U.K. |
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Abstract: | The seed globulins of Lupinus angustifolius are glycoproteins containing 1.4–1.9% (α-conglutin), 2.8–6.4 % (β-conglutin) and 1.2–3.8% (γ-conglutin) carbohydrate. The highest values were obtained after acid hydrolysis and determination by phenol—H2SO4, (α, γ-conglutins) or by methanolysis and sugar determination by GLC (β-conglutin). TCA denaturation of β- and γ-conglutins was necessary to remove adsorbed galactomannans before determination of glycoprotein carbohydrates. All 3 conglutins contained mannose, galactose and glucosamine, though the ratio of mannose to galactose, and to a lesser extent neutral sugars to hexosamine varied. Small amounts of fucose were found associated only with γ-conglutin. |
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Keywords: | Leguminosae seed glycoproteins carbohydrates. |
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