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Kinetics of fenugreek peroxidase isoenzymes
Authors:D. Balasimha  M.N. Tewari
Affiliation:Plant Biochemistry Laboratory, Department of Botany, University of Jodhpur, Jodhpur-342001, India
Abstract:Peroxidase from fenugreek seedlings was separated into 6 isoenzymes; 4 on CM-cellulose and 2 on DEAE-cellulose. The kinetics of these peroxidase isoenzymes with regard to o-dianisidine and catechol are described.
Keywords:Leguminosae  fenugreek  peroxidase isoenzymes  purification  catechol oxidation.  iodacetate, IAA  5,5′-dithiobis-(2-nitrobenzoic acid, DTNB  horseradish peroxidase, HRP.
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