Biosynthesis of N2-(1,3-dicarboxypropyl) ornithine [nopalinic acid] in crown gall tumour tissue |
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| Authors: | Ian M. Scott John L. Firmin |
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| Affiliation: | John Innes Institute, Norwich, NR4 7UH, U.K. |
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| Abstract: | Protein extract from crown gall tumour tissue, induced on Nicotiana tabacum by Agrobacterium tumefaciens strain T37, synthesized nopalinic acid [N2-(1,3-dicarboxypropyl)ornithine] from l-ornithine and α-ketoglutarate in the presence of NADPH. Label was incorporated into nopalinic acid from both l-ornithine-[14C] and α-ketoglutarate-[14C] in vivo. Nopaline [N2-(1,3-dicarboxypropyl)arginine] did not appear to be metabolized to nopalinic acid in vivo. |
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| Keywords: | Solanaceae crown gall tumours biosynthesis amino acid derivatives nopalinic acid. |
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