Purification of cassava linamarase |
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Authors: | Rodney D. Cooke Graham G. Blake Jon M. Battershill |
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Affiliation: | Tropical Products Institute, 56/62 Gray''s Inn Road, London WC1X 8LU, U.K. |
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Abstract: | Linamarase was purified from parenchymal tissue of cassava by extraction with acetate buffer, fractional precipitation with ammonium sulphate, followed by column chromatography on DEAE-cellulose and Sepharose-6-B gel filtration. The specific activity is increased 350 fold with 35% recovery. The Kms for linamarin and p-nitro-phenyl β-D-glucoside are 1.45 × 10?3 M and 0.46 × 10?3 M, respectively. The pH optimum in 50 mM NaPi is pH 6 and the specific activity is 26.5 nkat/mg. The enzyme can be prepared from cassava peel using the same procedure and has similar properties. |
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Keywords: | Euphorbiaceae cassava linamarase β-glucosidase purification. |
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