Purification of cassava linamarase

Linamarase was purified from parenchymal tissue of cassava by extraction with acetate buffer, fractional precipitation with ammonium sulphate, followed by column chromatography on DEAE-cellulose and Sepharose-6-B gel filtration. The specific activity is increased 350 fold with 35% recovery. The K_ms for linamarin and p-nitro-phenyl β-D-glucoside are 1.45 × 10^?3 M and 0.46 × 10^?3 M, respectively. The pH optimum in 50 mM NaPi is pH 6 and the specific activity is 26.5 nkat/mg. The enzyme can be prepared from cassava peel using the same procedure and has similar properties.