Amo 1618 effects on incorporation of 14C-MVA and 14C-acetate into sterols in Nicotiana and Digitalis seedlings and cell-free preparations from Nicotiana |
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Authors: | TJ Douglas LG Paleg |
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Institution: | Department of Plant Physiology, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, South Australia 5064 Australia |
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Abstract: | Incorporation of radioactivity from acetate-14C] and MVA-14C] into sterols and sterol precursors in tobacco was inhibited by Amo 1618; differing patterns of accumulation were obtained with the two precursors, suggesting more than one point of inhibition. This was borne out with cell-free preparations with which it was demonstrated that both HMG-CoA reductase and squalene-2,3-epoxide cyclase were inhibited, the latter more strongly than the former. GLC analysis of gross sterol and hydrocarbon fractions confirmed previous indications that incorporation of radioactivity into individual sterols was inhibited by Amo 1618. Finally, incorporation of MVA-14C] into sterols and sterol precursors of Digitalis was significantly altered by the retardant, thus expanding the generality of the relationship between sterol (particularly 4-desmethylsterol) biosynthesis inhibition and retardant effect. |
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Keywords: | Solanaceae cell free homogenates sterol biosynthesis HMG-CoA reductase squalene-2 3-epoxide AMO 1618 |
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