Synthesis of 5-aminolevulinic acid-[14C] by cell-free preparations from greening maize leaves |
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| Authors: | Eitan Harel Erna Meller Moshe Rosenberg |
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| Affiliation: | Botany Department, The Hebrew University of Jerusalem, Israel |
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| Abstract: | The conversion of 2-ketoglutarate-[14C] to 5-aminolevulinic acid-[14C] (ALA) by a cell-free system from maize leaves is described. Optimal conversion was achieved at pH 6.2 using a 20 000 g supernatant after gel filtration through Sephadex G-25. The formation of ALA required Mg2+, an amino donor (alanine or glutamate), pyridoxal phosphate and NADH. NADPH was somewhat less effective. |
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| Keywords: | Gramineae maize 5-aminolevulinic acid greening leaves chlorophyll. |
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