Degradation of homovanillate by a strain of Variovorax paradoxus via ring hydroxylation |
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Authors: | Nigel Allison Janet E Turner Robin Wait |
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Institution: | Centre for Applied Microbiology and Research, Porton Down, Salisbury SP4 OJG, UK |
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Abstract: | Abstract A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalysed a typical monooxygenase reaction and had little activity towards 4-hydroxyphenylacetic acid. GC-MS and TLC analysis suggested that homovanillate was 1-hydroxylated to yield a dihydroxymonomethoxyphenylacetic acid which served as a substrate for homogentisate 1,2-dioxygenase. Methanol, but not formaldehyde, was released either during ring-cleavage or subsequent metabolism of the ring-cleavage product. |
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Keywords: | Hydroxylase Biodegradation Methoxylated aromatic acid Ring hydroxylation Variovorax paradoxus |
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