A plate method to screen for microorganisms producing xylose isomerase |
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| Authors: | Sapunova L I Lobanok A G Kazakevich I O Evtushenkov A N |
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| Affiliation: | Institute of Microbiology, National Academy of Sciences of Belarus, Minsk, 220141 Belarus. enzyme@mbio.bas-net.by |
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| Abstract: | A plate method was developed to screen for xylose isomerase-producing microorganisms based on the use of 2,3,5-triphenyltetrazolium as an indicator of D-xylulose, the D-xylose isomerization product. The use of this method allows microorganisms to be differentiated by the character of the enzyme synthesis (inducible or constitutive). |
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