Sequencing bias: comparison of different protocols of MicroRNA library construction |
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| Authors: | Geng Tian XuYang Yin Hong Luo XiaoHong Xu Lars Bolund XiuQing Zhang |
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| Institution: | (1) Beijing Institute of Genomics, Chinese Academy of Science, 101300 Beijing, China;(2) The Graduate University of Chinese Academy of Sciences, 100062 Beijing, China;(3) Genome Research Institute, ShenZhen University Medical School, 518000 ShenZhen, China;(4) Beijing Genomics Institute, 518000 Shenzhen, China;(5) Insitute of Human Genetics, University of Aarhus, DK-8000 Aarhus, Denmark |
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| Abstract: | Background MicroRNAs(miRNAs) are 18-25 nt small RNAs playing critical roles in many biological processes. The majority of known miRNAs
were discovered by conventional cloning and a Sanger sequencing approach. The next-generation sequencing (NGS) technologies
enable in-depth characterization of the global repertoire of miRNAs, and different protocols for miRNA library construction
have been developed. However, the possible bias between the relative expression levels and sequences introduced by different
protocols of library preparation have rarely been explored. |
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