Primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, <Emphasis Type="Italic">Scylla paramamosain</Emphasis> |
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Authors: | Yan Xu Haihui Ye Jun Ma Huiyang Huang Guizhong Wang |
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Institution: | (1) Department of Oceanography, Xiamen University, Xiamen, 361005, China;(2) Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA; |
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Abstract: | Crustacean neurons, obtained from the cerebral ganglion of the mud crab Scylla paramamosain, were successfully cultured in vitro. They maintained typical morphological characteristics and showed better outgrowth in
modified Medium 199 (M199) medium than that in Liebowitz’s L-15 medium. Fetal bovine serum (FBS), muscle extracts, and hemolymph
of the mud crab S. paramamosain were added as supplements. Only 20% FBS could promote neuron outgrowth, while muscle extracts and hemolymph of S. paramamosain did not improve neuron outgrowth. For cell dissociation, both collagenase type I and trypsin worked well as determined by
initial cell viability and following cell outgrowth potential. More than six kinds of cells with different morphological characteristics
were identified in the neuron outgrowth. They were “small cells”, “veilers”, “branchers”, “multipolar cells”, “super-large
cell”, and “bipolar cells”. Among all of the cells, bipolar cells were identified for the first time in crustacean neurons
culture and they could live longer than other cells. The neurons could grow for more than a week before retraction and eventual
degradation. |
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Keywords: | |
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