Simultaneous measurement of unbound testosterone and estradiol fractions in undiluted plasma at 37 degrees C by steady-state gel filtration |
| |
Authors: | R A Fisher D C Anderson C W Burke |
| |
Affiliation: | Departments of Chemical Pathology and the Medical Professorial Unit, St. Bartholomew''s Hospital, London, E.C. 1.England;Endocrinology Service, Radcliffe Infirmary, Oxford, England |
| |
Abstract: | A method is described for the simultaneous measurement of percent unbound testosterone (T) and estradiol (E2) using steady-state gel filtration (SSGF), a technique which maintains physiological conditions of pH, temperature and plasma constituents, thus avoiding alterations in the original equilibria. Combined with specific assays of their total plasma levels it probably yields a closer estimate of the true circulating unbound (biologically-active) concentration of T and E2 than previous methods. Its main application lies in the study of sex-hormone disorders which may result in an imbalance between unbound T and E2 concentrations. Results of percent unbound are: normal men - T, 1.71 ± 0.29 (SD); E2, 1.79 ± 0.32: normal women - T, 1.02 ± 0.17; E2, 1.49 ± 0.35: hirsute women - T, 1.68 ± 0.52; E2, 1.55 ± 0.29; women on estrogens - T, 0.67 ± 0.06; E2, 1.02 ± 0.13. As predicted from earlier studies in hirsute females the fall in SHBG level is associated with a significant rise in percent unbound T, but no rise in percent unbound E2. |
| |
Keywords: | Steroids Aldosterone 18 11-Hemiacetal of 11β 21-dihydroxy-3 20-dioxo-4-pregnen-18-al. Androstenedione 4-Androstene-3 17-dione Cortisol 11β 17α 21-Trihydroxy-4-pregnene-3 20-dione. 11-deoxycortisol 17α 21-Dihydroxy-4-pregnene-3 1 3 5 (10)-Estratriene-3 17β-diol. Testosterone (T) 17β-Hydroxy-4-androsten-3-one. Binding Proteins Corticosteroid-binding globulin (CBG) Sex-hormone-binding globulin (SHBG) also known as testosterone-estradiol binding globulin (TeBG) |
本文献已被 ScienceDirect 等数据库收录! |
|