Analysis of Genetic Diversity at the arom Locus in Isolates of Pneumocystis carinii |
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| Authors: | SUNEALE BANERJI ELENA B LUGLI ROBERT F MILLER ANN E WAKEFIELD |
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| Institution: | Molecular Infectious Diseases Group, Department of Paediatrics, Institute of Molecular Medicine, John Radcliffe Hospital, OX3 9DU Oxford, United Kingdom;Department of Medicine, University College Hospital London Medical School and Camden &Islington Community Services (NHS) Trust, Middlesex Hospital Site, London, United Kingdom |
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| Abstract: | ABSTRACT. The DNA sequences of a portion of the 5-enolpyruvyl shikimate phosphate synthase domain of the arom gene, encoding the pentafunctional AROM protein, were determined from isolates of Pneumocystis carinii from five mammalian host species (rat, human, ferret, rabbit and mouse). High levels of genetic divergence were found among P. carinii derived from different hosts species, 7–22% at the DNA sequence level, and 7–26% at the derived amino acid sequence level. Two separate and distinct sequences were isolated from infected ferret lungs. Low levels of divergence were seen in human-derived organisms. |
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| Keywords: | Amino acid sequence AROM protein DNA sequence |
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