Metabolically engineered male sterility in rapeseed (<Emphasis Type="Italic">Brassica napus</Emphasis> L.) |
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Authors: | Thomas Engelke J Hirsche T Roitsch |
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Institution: | 1.Lehrstuhl für Pharmazeutische Biologie, Julius von Sachs Institut,Universit?t Würzburg,Würzburg,Germany;2.Institut für Pflanzenwissenschaften,Graz,Austria |
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Abstract: | Male sterility is of special interest as a mechanism allowing hybrid breeding, especially in important crops such as rapeseed
(Brassica napus). Male sterile plants are also suggested to be used as a biological safety method to prevent the spread of transgenes, a
risk that is high in the case of rapeseed due to the mode of pollination, out-crossing by wind or insects, and the presence
of related, cross-pollinating species in the surrounding ecosystem in Europe. Different natural occurring male sterilities
and alloplasmic forms have been tried to be used in rapeseed with more or less success. Due to the difficulties and limitations
with these systems, we present a biotechnological alternative: a metabolically engineered male sterility caused by interference
with anther-specific cell wall-bound invertase. This is an essential enzyme for carbohydrate supply of the symplastically
isolated pollen. The activity of this enzyme is reduced either by antisense interference or by expressing an invertase inhibitor
under control of the anther-specific promoter of the invertase with the consequence of a strong decrease of pollen germination
ability. |
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