Acridine orange staining of the mammalian fibroblast cell coat |
| |
Authors: | J Timár G Gyapay K Lapis |
| |
Institution: | (1) I. Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University Budapest, Üll i ut 26, H-1085 Budapest, Hungary |
| |
Abstract: | Summary Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compunds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric-in vitro-measurements and enzymatic digestions, respectively. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|