Evidence for a pertussis toxin-sensitive G protein involved in the control of meiotic reinitiation of Xenopus laevis oocytes |
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Authors: | V Pellaz S Schorderet-Slatkine |
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Affiliation: | Department of Obstetrics and Gynecology, H?pital Cantonal Universitaire, Geneva, Switzerland. |
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Abstract: | Meiotic reinitiation of Xenopus laevis oocytes is induced in vitro by progesterone or insulin. The hormonal effect is inhibited in a dose-dependent manner by the injection of the A protomer of pertussis toxin (islet-activating protein, IAP) into the oocytes. This inhibition occurs only before the appearance of a maturation-promoting activity in the cytoplasm. Furthermore, injection of the toxin A protomer into recipient oocytes does not inhibit the induction of maturation obtained through injection of cytoplasm containing the maturation-promoting factor. The inhibition effect of the toxin A protomer is reversible with time. These results suggest that a pertussis-sensitive G protein is involved in intracellular signaling systems leading to the induction of MPF activity. |
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