Immunoprophylactic studies on cell wall associated proteins ofMycobacterium tuberculosis H37Ra |
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Authors: | Neelam Dhiman Indu Verma G. K. Khuller |
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Affiliation: | (1) Department of Biochemistry, Postgraduate Institute of Medical Education and Research, 160 012 Chandigarh, India |
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Abstract: | The cell wall protein peptidoglycan complex (CW-PPC) of Mycobacterium tuberculosis H37Ra was isolated through sequential extraction of lipids, carbohydrates and soluble proteins. CW-PPC emulsified in FIA was found to induce significant protection in mice against challenge with LD50 dose ofM. tuberculosis H37Rv. To identify the immunoprotective components of CW-PPC, the proteins in avid association with peptidogican were dissociated by chemical treatment with trifluoromethanesulthonic acid (CF3CO3H): anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e., 71, 60 and 45 kDa proteins of cell wall was studied in animals immunized with CW-Pr-FIA. The 71 kDa protein was found to be most immunoreactive giving higher T-cell sensitization and humoral responses. Further, immunization of mice with 71 kDa-FIA demonstrated enhanced T- and B- cell responses. Mice immunized with 71 kDa-FIA gave significantly higher protection (P ≤ 0.05) against intravenous challenge with LD50 dose ofM. tuberculosis H37Rv, than BCG immunized animals. The results indicate the potential of 71 kDa cell wall protein as a suitable candidate for Cthe subunit vaccine. |
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Keywords: | Tuberculosis cell wall protein antigens vaccine |
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