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Assignment of sockeye salmon (Oncorhynchus nerka) to spawning sites using DNA markers |
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| Authors: | Graham E Corley-Smith Liv Wennerberg Joy A Schembri Chinten J Lim Karen L Cooper Bruce P Brandhorst |
| Institution: | (1) Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada;(2) National Centre for Biosystematics, Natural History Museums and Botanical Garden, University of Oslo, P.O. Box 1172, Blindern, NO-0318, Oslo, Norway;(3) Triton Environmental Consultants Limited, 150-13091 Vanier Place, Richmond, BC, Canada;(4) Present address: Environmental and Molecular Toxicology, Oregon State University, 1007 Agricultural and Life Sciences Building, Corvallis, OR, 97331-2914, U.S.A.;(5) Present address: Stressgen Biotechnologies Corporation, 350-4243 Glanford Avenue, Victoria, BC, V8Z 4B9, Canada;(6) Present address: 364 Oregon Hall, University of Oregon, Eugene, OR, 97403, U.S.A. |
| Abstract: | Randomly amplified polymorphic DNA (RAPD) markers were used to assign individual adult sockeye salmon to their spawning sites using a genotype assignment test. Six primers were selected for use by screening bulked DNA samples for markers missing in fish from one or more of 5 sites in British Columbia or Alaska. Of 73 markers scored, 54 showed variation between or within sites among the sampled fish. Thirty-seven of the variable markers were not detected in any fish from one or more sites; 18 variable markers were detected in all fish from one or more other sites. Thus 25% of markers scored were found in all fish of some sites and in no fish of some other sites. An assignment test placed all 70 fish tested into their correct populations. Principal coordinate analysis of genetic variation produced clusters of fish corresponding to each sampling site. No sex-specific RAPD markers were detected among more than 1300 screened. |
| Keywords: | assignment test sockeye salmon RAPD bulked DNA principal coordinate analysis sex-specific DNA markers |
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