| 灰树花漆酶酶学性质的初步研究 |
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| 引用本文： | 赵晓燕,邢增涛,邵毅,刘海燕,汪学才. 灰树花漆酶酶学性质的初步研究[J]. 天然产物研究与开发, 2012, 24(6): 824-827 |
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| 作者姓名： | 赵晓燕 邢增涛 邵毅 刘海燕 汪学才 |
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| 作者单位： | 1. 上海市农业科学院农产品质量标准与检测技术研究所;农业部食用菌产品质量监督检验测试中心,上海201106
2. 上海市农产品质量安全中心,上海,200030 |
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| 基金项目： | 上海市科技兴农重点攻关项目 |
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| 摘 要： | 漆酶是一种具有广泛应用前景的多功能酶,但能否大规模工业化生产成了制约其应用开发的主要瓶颈之一。担子菌类真菌是分泌漆酶的主要来源之一,灰树花隶属于担子菌,但有关其分泌漆酶的研究鲜有报道。本文初步探讨了pH值、温度及不同阴、阳离子等因素对灰树花孢外漆酶酶学特性的影响,结果表明灰树花孢外漆酶最适反应pH值为2.2,最适反应温度65℃,且反应体系中缓冲液的组成也会影响灰树花漆酶的活性。灰树花漆酶具有较强的热稳定性,但反应体系中若存在卤族离子则会强烈抑制灰树花漆酶的活性。本研究为今后开发利用灰树花漆酶提供了借鉴。
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| 关 键 词： | 灰树花 漆酶 pH值 温度 阴离子 阳离子 |
Preliminary Study on Enzyme Property ofGrifola frondosa Extracellular Laccase |
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| ZHAO Xiao-yan , XING Zeng-tao , SHAO Yi , LIU Hai-yan , WANG Xue-cai. Preliminary Study on Enzyme Property ofGrifola frondosa Extracellular Laccase[J]. Natural Product Research and Development, 2012, 24(6): 824-827 |
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| Authors: | ZHAO Xiao-yan XING Zeng-tao SHAO Yi LIU Hai-yan WANG Xue-cai |
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| Affiliation: | 1Institute for Agro-food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences; 2Supervision and Testing Center for Edible Fungi Quality,Ministry of Agriculture of China,Shanghai 201106,China; 3Agricultural Production Authentication Centre,Shanghai 200030,China |
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| Abstract: | Laccase is a kind of multifunctional enzyme which has extensive applied foreground,but output-lacking becomes a major problem for its use.Basidiomycota the major source for laccase secretion.This paper studied the property of extracellular laccase in cultures of Grifola frondosa which belonged to Basidiomycota fungi.The optimal temperature and pH value for laccase activity were 65 ℃ and pH 2.2,respectively.Enzyme activity was also affected by buffer composition.G.frondosa laccase was relatively heat stable.Halide ions could strongly inhibit laccase activity. |
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| Keywords: | Grifola frondosa extracellular laccase pH value temperature anions cations |
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