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How to predict product yield in kinetically controlled enzymatic peptide synthesis |
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| Authors: | Gololobov M Y Kozlova E V Borisov I L Schellenberger U Schellenberger V Jakubke H D |
| Affiliation: | Institute for Genetics and Selection of Industrial Microorganisms, 1st Dorozhny 1, Moscow 113545, Russia. |
| Abstract: | The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH(2) from Mal-L-Phe-L-AlaOMe and L-LeuNH(2). The experimental results demonstrate that this theory adequately describes the real process. (c) 1992 John Wiley & Sons, Inc. |
| Keywords: | theory of enzymatic peptide synthesis progress curve kinetics papain-catalyzed peptide synthesis |
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