Mosaic individuals found in genetically manipulated northern pike (Esox lucius) using flow cytometry |
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Authors: | Lin,Dabrowski,Luczynski,& Luczynski |
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Affiliation: | School of Natural Resources, The Ohio State University, Columbus, Ohio, USA;Inland Fisheries Institute, Olsztyn, Poland;Department of Basic Fishery Science, University of Agriculture and Technology, Olsztyn, Poland |
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Abstract: | The purpose of this study was to investigate abnormal larvae produced in the experimental induction of gynogenetic northern pike ( Esox lucius L.), using a flow cytometry method. We reported for the first time the mosaic individuals found in genetically manipulated northern pike. Two types of abnormal larvae were obtained after eggs were fertilized with UV-irradiated sperm and then exposed to heat shock treatment. One type had malformations frequently associated with haploid syndrome and were proven to be haploids. The other type had a similar body size and appearance as diploids but with curved body and a swirling swimming pattern. Individuals of the second type were proven to be mosaics containing both 1n (59.5 ± 14.7% mean ± SD) and 2n (29.7 ± 13.3%) cell populations. The ratio of 1n:2n cell populations ranged from 80.6:10.6 to 38.5:44.7. There was no significant difference in the relative nuclear DNA content and cell size between 1n and 2n cell populations of the mosaic individuals as compared to haploid cells and diploid cells, respectively. The nuclear DNA content of the northern pike was estimated to be 2.07 ± 0.06 pg ( n = 10) given that rainbow trout has a red blood cell nucleus DNA content of 4.66 pg. |
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