Genetic diversity of <Emphasis Type="Italic">Macrophomina phaseolina</Emphasis> isolates from certain agro-climatic regions of India by using RAPD markers |
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Authors: | Bandamaravuri Kishore Babu S S Reddy Mukesh K Yadav M Sukumar Vijendra Mishra A K Saxena Dilip K Arora |
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Institution: | 1.National Bureau of Agriculturally Important Microorganisms (NBAIM),Mau, Uttar Pradesh,India;2.Dept. of Microbiology,Bundelkhand University,Jhansi, Uttar Pradesh,India |
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Abstract: | Genetic diversity analysis of Macrophomina phaseolina isolates obtained from different host range and diverse geographical locations in India was carried out using RAPD fingerprinting.
Of the thirteen 10-mer random primers used, primer OPB-08 gave the maximum polymorphism and the UPGMA clustering could separate
50 isolates in to ten groups at more than 65% similarity level. The ten clusters correlated well with the geographical locations
with exceptions for isolates obtained from Eastern and Western Ghats. There was a segregation of isolates from these two geographical
locations in to two clusters thus, distributing 10 genotypes in to eight geographical locations. All the isolates M. phaseolina irrespective of their host and geographical origin, exhibited two representative monomorphic bands at 250 bp and 1 kb, presence
of these bands suggests that isolates might have evolved from a common ancestor but due to geographical isolation fallowed
by natural selection and genetic drift might have segregated in to subpopulations. Genetic similarity in the pathogenic population
reflects the dispersal of single lineage in all locations in India. |
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Keywords: | Macrophomina phaseolina Random amplified polymorphic DNA Genetic diversity |
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