| 仙台病毒BB1株基因组cDNA序列测定及比较分析 |
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| 引用本文： | 杨宇,任鲁风,张辉,侯云德,吴小兵.仙台病毒BB1株基因组cDNA序列测定及比较分析[J].病毒学报,2006,22(2):96-100. |
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| 作者姓名： | 杨宇 任鲁风 张辉 侯云德 吴小兵 |
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| 作者单位： | 1. 中国疾病预防控制中心,病毒基因工程国家重点实验室,北京,100052
2. 中国科学院研究生院,北京,100039 |
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| 基金项目： | 国家973项目资助（2004CB518806） |
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| 摘 要： | 该研究对仙台病毒BB1分离株的cDNA全序列进行测序,通过RT-PCR法获得的4个相互重叠的质粒克隆覆盖了全长基因组,并将BB1全长序列与其它已知的仙台病毒序列进行比较。BB1株病毒的基因组为15 384个碱基构成,与其它已知仙台病毒基因组的基因排列与组成规律是一致的,未发现插入或缺失突变。与现已公布5个仙台病毒代表株全基因组序列比较发现,BB1株与其它仙台病毒株同源性均有较大差异。遗传进化分析结果显示,BB1株与Z株和Hamamatsu株的同源性仅为87%和91%,不属于这两株代表的进化分支而归属于第三个基因型。
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| 关 键 词： | 仙台病毒 基因组 核苷酸序列分析 |
| 文章编号： | 1000-8721（2006）02-0096-05 |
| 收稿时间： | 2005-10-21 |
| 修稿时间： | 2005-10-212005-12-22 |
The Complete cDNA Sequence of Sendai Virus Isolate BB1 and Comparison with the Other Isolates |
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| YANG Yu,REN Lu-feng,ZHANG Hui,HOU Yun-de,WU Xiao-bing.The Complete cDNA Sequence of Sendai Virus Isolate BB1 and Comparison with the Other Isolates[J].Chinese Journal of Virology,2006,22(2):96-100. |
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| Authors: | YANG Yu REN Lu-feng ZHANG Hui HOU Yun-de WU Xiao-bing |
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| Institution: | 1. State Key Laboratory for Molecular Virology and Genetic Engineering, China CDC, Beijing 100052, China ; 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | In this study,a novel Sendai virus(SeV)isolate BB1 strain was fully sequenced,four overlapping clones covering the whole viral genome were obtained by RT-PCR,the entire nucleotide sequence of the BB1 strain was determined and compared with that of the other known sequenced isolates of Sendai virus.The viral genome of BB1 isolate comprises 15 384 nucleotides,which are conserved in all of the SeVs.BB1 deduced a homologous genome organization of all SeVs,no insertion or deletion is detected in the coding regions.Sequence comparisons with other 5 fully sequenced representative isolates revealed that BB1 isolate is not close to any published SeV sequences.The phylogenetic analysis demonstrates that BB1 strain shares low homology with Z strain(87%) and Hamamatsu strain(91%),BB1 is not like the above two evolutional lineages and belongs to a third different genetic group. |
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| Keywords: | Sendai virus genome nucleotide sequence analysis |
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