Sodium binding in Halobacterium halobium measured by the nuclear magnetic resonance technique |
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Authors: | Ruth Melamud Martin Risk H. Gilboa |
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Affiliation: | 1. Department of Chemistry, TECHNION, Israel Institute of Technology, Haifa 32000 Israel;2. Department of Biology, TECHNION, Israel Institute of Technology, Haifa 32000 Israel |
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Abstract: | Relaxation time measurements T1 and T2 of sodium in Halobacterium halobium pellets were carried out at two frequencies. From those measurements, combined with intensity measurements of the sodium in the system, estimation of the properties of the sodium ions in the system was carried out. It is suggested that three types of sodium ions are present in the bacterial pellet. (A) The extracellular sodium with properties of free solution and (B) sodium which is in the pericellular volume between the cell wall and the cell membrane. There is an exchange between type A and type B sodium. The type B sodium has (e2qQ)/h = 3.7 · 107 rad/s, τcB = 5.2 · 10−6 s and τB = 1 · 10−3 s. The sodium of type C is bound inside the cell and undetected. It's concentration inside the cell is assumed to be 1.9 M. |
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Keywords: | Na binding NMR (H. halobium) |
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